Abstract:

Extracting high-quality total RNA from walnut buds is a complicated and rigorous precess as the walnut tissue is rich in phenolic compounds、polysaccharides and other secondary metabolites, this may influence the follow-up molecular biology research. In this paper, we discussed different extraction method of Walnut buds and obtained a effective method. The results showed that added PVPP to the grinding and the use of modified SDS method of PH9.0 sodium borate buffer and the modified CTAB method can extract walnut buds RNA well,and we got the target fragment with the reaction of RT-PCR.So we can acquire the conclusion that our method about extracting walnut bud RNA is available to be used for the research ahout Walnut’s flowering gene .