Abstract: In order to cultivate grapes detoxification seedlings and further promote the study of grape tissue culture techniques, a number of factors affecting the vitro rapid propagation of grapes were tested and the culture medium most suitable for the grape in vitro rapid propagation was elected. 2-year-old branches at different states (dormant period, growing season) were taken as the material which was cut into 1.5 cm stem segments after disinfection in the ultra-clean work bench to be cultured to contrast. It is found that in spring, the winter buds of the branches after which are relieved dormancy has the highest sprout rate and survival rate and the best growth potential. The combination of growth of the culture medium of subculture multiplication MS+6-BA 1.0 mg/L+ IBA 0.1 mg/L had the maximum effective seedlings. The rooting culture medium, 1/2MS + IBA 0.4 mg/L, IBA was the most conducive to the rooting of grape, the seeding in this concentration had the highest survival rate. In the process of the experiment, the vitrification seeds occasionally appear in the optimum proliferation combination, therefore, the best subculture medium obtained in the experiment was just the best proportioning combination. A further study on that the concentration is adjusted plus or minus within the range of the best proportioning should be done. For rooting culture, 1/4MS, 1/6MS, 1/8MS try to be used for further pilot study.