Abstract:

In order to solve the difficult of isolation the algicidal bacteria, isolated algicidal bacteria with pectin as the sole carbon source in this experiment. To explore the relationship between the pectinase activity of the bacteria lysing bacteria, the lytic effect of lysing-bacteria on Microcystis aeruginosa and the pectinase activity were compared and analyzed. The results showed that five strains had lytic characteristics, the first day of the experiment, the cells of the Microcystis appeared yellow and bacteria film wrapped Microcystis cells sunk. Compare the five strains on the best lysing-condition, bacterial liquid and the algae liquid in volume ratio: w01> w03= w05> w04> w02; the speed: w05> w04> w01 = w02> w03; continuous lysing-algae time: w04> w05> w01> w02> w03. pectinase activity: w04> w01> w03> w02= w05. The medium with pectin as sole carbon source can be used to isolate algicidal bacteria effectively, however there no linear relationship between the lytic effects of bacterial pectinase activity.