Abstract:

Abstract: The purpose of the study is to develop 2-DE method suitable for maize leaf proteome analysis in future. Three different methods were adopted to extract leaf protein of maize line Qi319. And the prepared samples were separated by SDS-PAGE and two-dimensional electrophoresis, followed by Silver nitrate staining. The result shows that TCA/acetone precipitation method is suitable for extracting maize leaf protein. IEF method is carried out on 24cm IPG strips at pH 4-7 and protein sample weight 0.25 mg. IEF program: 100V 1 h; 300V 1h; 500 V 1 h; 1000 V 2 h; 8000 V 3 h; 8000V 10h. Condition of SDS-PAGE is 1 W/gel 1 h, 3W/gel to Bromophenol blue reaching bottom. The establishment of the suitable system for maize leaf proteomic research paves the way for the next step to further analyse maize drought-resistance at proteome level.