Abstract: Taking ‘Jinheding’ and ‘Chihui’ as parents, rootstocks were used as materials for studying the multiplication of rootstocks. There were including choices of basic medium, the effects of 6-BA and NAA on the multiplication. The result showed that the growth state was affected evidently by medium. 1/2MS medium was the basic medium for rootstocks of Cymbidium goeringii. 1/2MS+ NAA 1 mg/L+6-BA 0.5 mg/L was the best medium for multiplication. proliferation rate was 3.00. Cross breeding technology combining with tissue culture was the effective way for the new species breeding of Cymbidium.