Abstract: In order to establish the suitable disinfection method for tissue culture of Zoster marina. 75% ethanol, 1.5% potassium iodide, 4% sodium hypochlorite and 10% hydrogen peroxide as well as different disinfection time were used for seeds and seedlings sterilization treatment. The results indicated that for seedlings, sterile sea water rinsed for 3-4 times, 75% ethanol sterilized for 20 s, then sterile sea water rinsed for 3-4 times, which achieved good sterilizing effects. The efficient disinfection method for seeds was that, 75% ethanol rinsed for 10 min, 1.5% potassium iodide immersed for 10 min and then sterile sea water rinsed for 3-4 times, which showed the optimal disinfection effects and did not affect seed germination rate.