Abstract: The study on the distribution and morphology of broiler’s intestinal stem cells by immunohistochemical technology had been carried on, which would explore the relationship between intestinal stem cells and intestinal injury. In this experiment, the duodenum, jejunum and ileum of the 28 day old broilers was collected. Lgr5 was selected to the intestinal stem cell marker and the distribution and morphology characteristics of broiler intestinal stem cell was observed by immunohistochemical metho. The results showed that the specificity of Lgr5 as intestinal stem cells marker was strong. The intestinal stem cell was found in duodenum, ileum and jejunum of growing broiler, which were located in the +1 to the +4 position above paneth cells, and the cells were showed columnar or taperedconical, the nucleus were oval or blurring. The average number of intestinal stem cells in every microscope view was two in duodenum, three in ileum and three in jejunum. Therefore we confirmed that Lgr5 can be used as broiler intestinal stem cell markers, and the morphological structure of broiler intestinal stem cells was observed, the number of small intestine in the growing broiler was preliminarily determines, which will lay the foundation for further explore the role of intestinal stem cell in the growing broiler.