Abstract: Eshuiqin 1. was used as experimental material and peat, vermiculite, mushroom residue, soil were as substrate materials,The paper studied 8 different formulations of substrate cultivation of O. javanica (BI. ) DC.．The results showed that the formula with 50% peat and 50% mushroom residue got the highest yield，per 667m2 up to 6510.6kg，could be recommended for greenhouse substrate cultivation ideal formula of water dropwort．The test results of nutritional components showed that water dropwort planting in outdoor field had higher nutrient levels, however in greenhouse substrate culture less nutritional value, but more tender．