Abstract: The purpose of this study was to determine the proximate composition and collagen of fish muscles of Lateolabrax japonicas, Liza haematocheila, Ctencpharyngodon idellus, Cyprinus carpio, which analyzes its collagen content. The results showed that crude protein contents in these fish muscles were 18.23%, 19.82%, 16.18%, 17.07%. Hydroxyproline contents were 0.0574%, 0.0645%, 0.0781%, 0.0743%, collagen contents were 0.6371%, 0.7159%, 0.8669%, 0.8247%. The results showed that fish muscle was a good source of protein only from the protein content, and saltwater fish was better than freshwater fish. However, collagen of fish muscle was lower, indicating that fish muscle was not suitable as raw material for extraction of collagen.