Abstract: Both development of intensive livestock farming and widely use of feed additive in the livestock and poultry production increase greatly the content of Cu in livestock and poultry manure, which increase the environmental risk of livestock manure applied in farmland. To reduce the solubility of Cu in livestock and poultry manure, six common mineral materials, including montmorillonite, fly ash, alum, zeolite, diatomite and apatite, were selected to test their capacities for stabilization of Cu in pig manure. Sequential fractionation method of heavy metals and leaching experiment were applied to characterize changes in the chemical forms of Cu in pig manure, and evaluate the inactivation of different mineral materials on Cu in pig manure. The results showed that Cu in pig manure without addition of any mineral materials is mainly of organic bound and residual forms with higher percentage of exchangeable copper (5.87%). All of the mineral materials had significant inactivation on Cu in pig manure. As compared with the control, application of montmorillonite, fly ash, alum, zeolite, diatomite and apatite decreased the exchangeable Cu by 45.1%, 73.1%, 64.6%, 38.3%, 22.7%, and 68.3%, respectively. Addition of the minerals decreased significantly the leaching of Cu from pig manure. The inactivation on Cu in pig manure with application of the mineral materials may be related to the transformation of Cu forms from exchangeable to organic matter bound, oxide bound and residual.