Abstract: In order to screen out new flue-cured tobacco varieties adapted to the ecological conditions of Shaoyang City, taking‘K326’as the check variety, the comparative tests of 5 flue-cured tobacco varieties,including‘Yunyan99’,‘NC297’,‘NC196’,‘Yueyan99’and‘Bina 1’were carried out in Shaoyang, Hunan Province in 2013, their biological characters, economic characters and appearance qualities were observed and analyzed. The results showed that the yield and output value of‘Bina 1’,‘Yueyan99’and‘Yunyan97’were higher than other varieties; quality of‘NC297’,‘NC196’was worse. Except‘Yueyan99’, the effect of other flue-cured tobacco varieties was small. Comprehensively considering the yield and quality of these 5 varieties,‘Yunyan 99’and‘Bina 1’could be planted and demonstrated in Shaoyang City,‘Yueyan 99’needed further verify the disease resistance,‘NC297’and‘NC196’need optimize field management and flue technology.