Abstract: This review introduced the progress about application of biotechnology in the strawberry genetics and breeding from abroad and home researches in recent years. The role and the status of application about tissue culture technology, molecular markers and genetic engineering technology in the strawberry genetics and breeding were summarized mainly including the application of tissue culture in strawberry breeding, the using of molecular markers about the constructing of genetic maps and molecular marker-assisted selection for disease resistance, the using of genetic engineering technology about functional verification of strawberry genes and the directional genetic improvement of horticultural traits and so on. The feasibility of modern biotechnologies were applied to breeding research was analyzed as well. Finally, the limitations of various biotechnologies were summarized, the utilization way and development direction of modern biotechnology combined with conventional breeding technology was discussed. Overall, this review aimed at offering basic knowledge about genetic breeding work of strawberry.