Abstract: In order to improve the technology level of sugar beet fertilization, nutrient deficiency test of nitrogen, phosphorus, potassium, boron and zinc were designed in four areas of Xinjiang and Gansu Province to study the effect of fertilization on yield, sugar content, sugar yield and nutrient utilization rate. Results showed that the production of different treatments was between 58.95%-89.51%, full fertilizer treatment increased the yield and sugar yield. Sugar content was negatively correlated with yield, the influence of fertilization on yield was greater than sugar yield. Nutrient limiting factors restricting sugar beet yield were different among different areas, in Changji and Qitai, nutrient limiting factors were mainly phosphorus and potassium, followed by nitrogen; for experiment in Yili, the sequence was N>K>Zn>P>B; for experiment in Zhangye, the sequence was B>N>P>K>Zn. The average utilization rate of nitrogen, phosphorus and potassium was 38.3%, 15.38% and 34.13%, the result could provide reference for local balanced fertilization.