Abstract: Abstract: In order to select new flue-cured tobacco varieties (lines) suitable for Heilongjiang flue-cured tobacco areas, a comparative trail of seven new introduced flue-cured tobacco varieties (lines), including of ‘Youxuan1’, ‘LY0414’, ‘Y8190’, ‘0408’, ‘CF225’, ‘Y106’, ‘JZ7-07’, were identified, using ‘NC89’ as controls. The results showed that ‘Y8190’, ‘0408’ and ‘JZ7-07’ had favorable characters of yield, output value, quality, adaptability and economic efficacy, and the comprehensive characters of ‘Y8190’, ‘0408’ and ‘JZ7-07’ were superior to those of ‘NC89’ as check variety. They should be recommended for further demonstration in Heilongjiang flue-cured tobacco areas.