Abstract: In order to fully utilize mulberry twigs resources in silk production and expand sources of raw materials in edible fungus production, the mushroom cultivation experiments for Oudemansiella radicata with different methods and proportions of mulberry branch powder was studied. The mulberry twigs powder proportion was from 75%-80% in the formula of mushroom growth medium, the mycelia growth rate was faster than other media, it might reach the 1.96-2.63 mm/date. Oudemansiella radicata yield determination showed that, beginning from June of 2011 for the first yield fluctuation to September for the end, every 2 to 3 days harvests one time in the period. The fruit body growth and development was faster in the summer than other seasons, and higher yield and biological efficiency could reach 75%-84.4%, fruiting body was higher, thicker in pileus and larger and thicker for its caps. Economic analysis showed that 100 kg raw material could be obtain net economic income more than ￥300 , and as to expand the planting scale showed better economic benefits. The experimental results showed that the mulberry branches as a kind of raw materials for mushroom production was a best way of comprehensive utilization in mulberry and mushroom production, it saved the cost broad application prospects.