Abstract: A field experiment was conducted using split-plot experiment design i.e. three regimes of planting density as main plots and nitrogen rate as sub-plots to investigate the effects of interaction between nitrogen application rate and planting density on main growth time, agronomic characters, grain yield and quality of flue-cured tobacco to determine the optimum nitrogen application rate and planting density of ‘Liaoyan 17’. The results showed that both lower and upper leaf mature stage were put off under same nitrogen rate, with density increasing. In addition, the effects of different treatments on leaf area were larger, and under seam nitrogen rate conditions (exception to middle nitrogen group), lower leaf area increased first, and decreased later with density increasing. Under seam nitrogen rate conditions, yield, average price, output value, percentage of superior and medium tobacco decreased gradually, when density increased; with nitrogen rate increasing, yield increased, but average price declined gradually under same density conditions. On the consideration of comprehensive analysis, the responses from the nitrogen were greater than those from density and their interaction, and the appropriate density and nitrogen rate for ‘Liaoyan 17’ were 15000-17250 plants/hm2 and 60.0-75.0 kg/hm2 respectively, which can improve growth and development resulting in better yield and quality of flue-cured tobacco based on the interaction between density and nitrogen rate.