Abstract: In order to perfect the techniques of bee pollination for pear, improve the efficiency of bee pollination and save cost, research the influence of the introduced bee colonies in different florescence on the honeybees collecting pear pollen. Using Pyrus bretschneideri ‘Dangshansuli’ and Apis mellifera as test materials, study the influence of introduced bee colonies in different florescence on the effect of pear pollination. The results showed that there was a large quantity of pollen dispersal during pear declining period, the quantity of the pollen collected by bees was to maximum, there was a positive correlation between the pear pollen weight and flowering quantities of introduced bee colonies in the first day, the pear pollen percentage of introduced bee colonies which collected during early blooming time and full blooming time were higher than other florescence, the weight and percentage of pear pollen of introduced bee colonies which collected during early blooming time were higher than other time in the same day, the peak time of collecting pear pollen was between 12:00 to 13:00, the foraging ability and pollination efficiency of the bee colonies introduced during early blooming and full blooming time was better than other florescence. So we think that the bees should be introduced to pollinate when the flowering percentage is about to 20%, the effect of pear pollination could be the best.