Abstract: The experiment studied the change of immune-related enzyme and MDA content in rising temperature. The activities of SOD, CAT, POD, LZM and the content of MDA in family of sea urchins (Strongylocentrotus intermedius) with different color tube feet were analyzed. The result show that:(1)In rising temperature, the change of SOD,CAT,POD,LZM were trend to increased and to decreased in RR, RW, WR, WW family of sea urchins, the activities of SOD were 2.39 ~4.36U/ml, 2.79 ~5.17U/ml, 2.71 ~4.97U/ml,3.66 ~5.69U/ml respectively; the activities of CAT were 63.02 ~112.76U/ml, 62.47 ~169.37U/ml, 66.40 ~140.77U/ml, 72.29 ~149.20U/ml respectively; the activities of POD were 1.65 ~14.59U/ml, 0.89 ~13.88U/ml, 2.08 ~14.68U/ml, 2.49 ~15.62U/ml respectively; the activities of LZM were 9.82 ~109.23U/ml, 12.10 ~110.37U/ml, 7.82 ~112.16U/ml, 11.18 ~107.83U/ml respectively; the activity of MDA first reduced and then increased in RR, RW and WR family, the content of MDA were 0.97 ~2.34nmol/ml, 0.82 ~2.40nmol/ml, 0.96 ~2.23nmol/ml, the activity of MDA in WW family was trend to increased, the content of MDA was 1.55 ~2.60nmol/ml. (2)The change of SOD,CAT,POD,LZM were trend to increased and to decreased in red and white tube feet S. intermedius, the activity of MDA first reduced and then increased in red tube feet sea urchins, and increased in white tube feet sea urchins. (3) In rising temperature, the activity of SOD, CAT in RR family was more sensitive than WW family, the content of MDA in RR family was lower than in WW family; the activity of SOD, POD in red tube feet sea urchins was more sensitive than in white tube feet sea urchins(P<0.05); in 26℃ the activity of POD had significant difference(P<0.05). These result revealed that family of sea urchins presented in the aspect temperature sensitively and adaptability strongly whose foster parents were the red tube feet sea urchins.