Abstract: The aims were to study the effect of different planting density and fertilization level on the yield and component factors of ‘Cui Yi No.1’ after planting tobacco, and select the optimal combination of high yield cultivation. L16(45) orthogonal design randomized block arrangement field experiment method was used in the 32 m2 residential area. Field management was carried out according to the growth stage, and related growth data was collected, the harvest yield was checked and accepted. The results showed that, fertilization level, and both of them interaction on yield of ‘Cui Yi No.1’ had significant differences. In a certain range, with the increase of planting density and fertilization level increased, the yield was also increased correspondingly, but beyond the scope of dense planting, fertilization level was too high, the yield decreased., the direct contribution to the yield in the 4 factors of yield components, was as follows: the effective plant number> grain number per plant > setting seeds rate > 100 grain weight. There are obvious mutual influence relations among the effective plant number, grain number per plant, setting seeds rate, and 100 grain weight of ‘Cui Yi No.1’. Every hectare planting density 30000 plexus-36000 plexus with N 225 kg, P2O5 112.5 kg, k2O 225 kg collocation, adding other high-yield cultivation technique, the yield could reach more than 4600 kg.