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Journal of Agriculture ›› 2016, Vol. 6 ›› Issue (1): 56-61.doi: 10.11923/j.issn.2095-4050.cjas15060013

Special Issue: 生物技术

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Variation of Soil Nutrients, Microbial Biomass and Enzymatic Activity with Isoetes sinensis Palmer Growth Under Different Cultivation Conditions

  

  • Received:2015-06-11 Revised:2015-06-30 Accepted:2015-07-06 Online:2016-01-29 Published:2016-01-29

Abstract: In order to rejuvenate critically endangered Isoetes sinensis Palmer, and try to find out its suitable cultivation conditions, we studied the variation of microbial quantity, soil nutrient and enzyme activity after Isoetes sinensis Palmer growth under three artificial cultivation modes, i.e. constant temperature condition (CTC), greenhouse condition (GHC) and outdoor condition (ODC). The results showed that soil pH increased (P<0.05), the number of soil bacteria (BAC) reduced (P<0.05) under GC and OC, but the number of soil fungi (FUN) and actinomycetes (ACT) increased (P<0.05) under OC after Isoetes sinensis Palmer growth compared with that under the original soil condition. Soil total nitrogen (TN) and available phosphorus (AP) content, invertase (INT) and protease (PRO) activity decreased (P<0.05) under all conditions, while soil alkalihydrolysable nitrogen (AN), total phosphorus (TP) and organic matter (OM) content, phosphatase (ACP) and urease (UR) activity reduced (P<0.05) only under GC. Soil catalase (CAT) activity also reduced (P<0.05) only under CTC. The above showed that different cultivation conditions had different influences on soil microbes, nutrients and enzyme activity after Isoetes sinensis Palmer growth. So it was necessary to fertilize the soil reasonably and adjust the soil biological activity effectively after Isoetes sinensis Palmer growth.

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