Abstract: The purpose of the study was to select the optimal irradiation dosage, which was very important to improve the salt resistance of Glycyrrhiza glabra seed, and provided guidance for the development of artificial cultivation of the licorice in saline-alkali desert soil. Radiation induced mutation technology was used to investigate the germination pattern of Glycyrrhiza glabra seeds under severe salt stress after 60Co-γirradiation with different doses. Comparing the seed germination rate, cumulative germination rate and germination index under high concentration of Na2SO4 stress environment were compared with control. The results showed that a negative correlation between salt concentration and the Glycyrrhiza glabra seeds germination rate which without irradiated, with the increased of salt concentration, the germination rate significantly decreased, initial germination time delayed, and extended the time to get maximum germination rate. The seed which is treated by 100 Gy and 300 Gy 60Co-γ irradiation the germination rate was significantly higher than the seed which is no irradiation, and the germination time was significantly shorter under Na2SO4 stress environment. The appropriate doses of 60Co-γirradiation treatment could effectively improve the seeds germination of Glycyrrhiza glabra under severe salt stress conditions. In conclusion, seeds should be treated under certain doses of 60Co-γaccording to the concentration of Na2SO4 in soils before sowing.