Abstract: To solve the serious problem of Fusarium wilt and Verticillium wilt disease in cotton production, the varieties with rich genetic diversity and good comprehensive characters were selected as the female parent and the different resources with complementary properties were selected to cross with the female parent in every generation to improve the defect of the female parent. During the whole breeding progress, sister crossing was applied to increase the good genes accumulation rate and recombination rate. In the serious Fusarium wilt and Verticillium wilt disease nursery, the selection of high-yield was carried out. Through the identification of disease resistance in whole breeding progress, plants with low disease level and incidence were selected; according to the cotton quality supervision standard of MOA test center, the cotton variety was selected by national variety certification level; high- yield, high fiber quality cotton varieties with Fusarium wilt and Verticillium wilt resistance were bred such as‘Jimian616’and‘Jimian315’