Abstract: We aim to analyze the effects of vegetable carbon black at different levels on growth performance, digestive enzymes and non-specific immune activities of wild F1 juvenile Mastacembelus aculeatus. A basal diet without adding vegetable carbon black and three test diets containing 0.1%, 0.5% and 1.0% vegetable carbon black were prepared to feed four groups of M. aculeatus in triplicate respectively. The feeding experiment was conducted in 12 tank systems (150 L each) at a controlled water temperature of 26-28℃, dissolved oxygen of 5.0-6.0 mg/L and pH 6.5-7.5 for eight weeks. The results showed that compared with the control group, the weight gain rate and specific growth rate of 0.5% vegetable carbon black group significantly increased by 24.76% and 16.18% respectively (P<0.05), and feed conversion rate of 0.1% vegetable carbon black group significantly reduced by 30.23% (P<0.05). Survival rate was not affected by vegetable carbon black (P>0.05). The activity of pepsin was the highest in the group of 0.5% vegetable carbon black (P<0.05). The activities of amylase in both intestine and liver first increased and then decreased with the increase of vegetable carbon black, with the highest value in the group of 0.1% vegetable carbon black (P<0.05). In the respect of non-specific immunity, the addition of 0.1% and 0.5% vegetable carbon black in diet had significant effect on activities of SOD, CAT, ACP, AKP in intestine (P<0.05). And the CAT activity in liver was the highest in the group of 0.1% vegetable carbon black (P<0.05). But SOD, AKP, ACP activities in liver were no affected in all the tested groups (P>0.05). It can be concluded that the growth performance and the activities of enzymes in M. aculeatus could be improved by adding 0.1%-0.5% vegetable carbon black.