Abstract: The objective of this experiment is to study the effects of row spacing and density on the yield and quality of new bean variety‘Henong76’, and clarify the optimum planting density at different row spacing. Split spot design was conducted in the research with row spacing as the main plot, including 30 cm and 45 cm row spacing, planting density was as the sub-plot including 300000, 350000, 400000, 450000, and 500000 plants/hm2, identified as D1, D2, D3, D4, and D5, respectively. The results showed that soybean yield first increased, and then decreased with the increasing planting density. D2 treatment (350000 plants/hm2) had the highest yield compared with other treatments. Moreover, there was no lodging problem for‘Henong76’under D2 treatment. The optimum planting density was 380000 plants/hm2 at 45 cm row spacing, and the yield was 3289.79 kg/hm2. The optimum planting density was 371800 plants/hm2 at 30 cm row spacing, and the yield was 3211.96 kg/hm2. The contents of protein and fat decreased along with the increasing planting density. D1 treatment obtained the highest content of protein and fat. Taken together, for‘Henong76’, the optimal planting density was 380000 plants/hm2 at 45 cm row spacing, or 371800 plants/hm2 at 30 cm row spacing.