Principal Component Analysis of the Effect of Different NaCl Concentrations on ‘Tamina’Grape Seedlings

doi:10.11923/j.issn.2095-4050.cjas17060014

Abstract

Abstract: The paper aims to select the main indexes of the effects of NaCl stress on grapevine and provide theory basis for evaluation the salt resistance system of grapevines. With the annual of grapes ‘Tamina’ grown on the sand as the experimental material, six concentration of NaCl treatments were conducted, then the relative conductivity of grape root and leaf, the oxygen anion production rate, H2O2 content, antioxidant enzyme (SOD, CAT, POD, APX) activity of leaves, shoot length and total biomass of plants were analyzed. Results showed that the root and leaf relative conductivity increased gradually with the increase of NaCl concentration compared with the control, the super oxygen anion production rate and H2O2 content increased, SOD and CAT activity in the leaves presented a trend of rise-down, APX activity presented a trend of down-rise-down, while POD activity presented a downward trend; under high concentrations of NaCl treatment, the length of shoot and total biomass of the plants were reduced, higher oxygen anion production rate and H2O2 content in grape leaves might be related with the lower antioxidant enzyme activity. The principal component analysis indicated that the shoot length, CAT activity, H2O2 content and APX activity of leaves could be used to evaluate the effects of different concentration of NaCl stress on grapevine.

CLC Number:

S663.1

References

[1] Hasegawa PM, Bressan RA, Zhu JK, et al. Plant cellular and molecular responses to high salinity [J]. Annu Rev Plant Physiol Plant Mol Biol, 2000, 51: 463-499.
[2] 许祥明, 叶和春, 李国凤. 植物抗盐机理的研究进展[J]. 应用与环境生物学报, 2000, 6 (4): 379-387.
[3] 杨劲松. 中国盐渍土研究的发展历程与展望[J]. 土壤学报, 2008, 45 (5): 837–845.
[4] 任玉华. 葡萄耐盐性研究进展[J] . 中外葡萄与葡萄酒, 2005, (4) : 31- 33.
[5] Levitt J. Responses of plant to environmental stresses. Vol II Water,radiat ion, salt, and other stresses[ M] . New York: Academic Press, 1980: 365 -490.
[6] 樊秀彩, 张亚冰, 刘崇怀, 等. NaCl胁迫对葡萄幼苗叶片有机渗透调节物质和膜脂过氧化的影响[J]. 果树学报, 2007, 24(6): 765-769.
[7] 徐海, 张红云, 景学芳. 几个鲜食葡萄品种抗盐性比较研究[J]. 中外葡萄与葡萄酒, 2010(1): 25-27.
[8] 廖祥儒, 贺普超, 万恰震. 盐胁迫对葡萄新梢叶片的伤害作用[J]. 果树科学, 1996, 13(4): 211-214.
[9] Hatami E, Esna-ashari M, Javadi T. Effect of salinity on some gas exchange characteristics of grape(Vitis vinifera) cultivars[J]. International Journal of Agriculture and Biology, 2010, 12: 308-310.
[10] 邢庆振, 郁松林, 牛雅萍, 等. 盐胁迫对葡萄幼苗光合及叶绿素荧光特性的影响[J]. 干旱地区农业研究, 2011, 29(3):96-100.
[11] 秦玲, 康文怀, 齐艳玲, 等. 盐胁迫对酿酒葡萄叶片细胞结构及光合特性的影响[J]. 中国农业科学, 2012, 45(20):4233-4241.
[12] 郝玉杰, 武林楠, 冯建荣, 等. NaCl 胁迫对两个葡萄品种叶片光合特性的影响[J]. 新疆农业科学, 2016, 53(10) : 1794 -1800.
[13] 李学孚, 倪智敏, 吴月燕, 等. 盐胁迫对‘鄞红’葡萄光合特性及叶片细胞结构的影响[J]. 生态学报, 2015, 35(13):4436-4444
[14] 沈渊. SPSS17.0(中文版)统计分析及应用实验教程[M]. 杭州:浙江大学出版社, 2013:8.
[15] 王依, 靳娟, 罗强勇, 等. 4 个酿酒葡萄品种抗寒性的比较[J]. 果树学报, 2015, 32(4): 612-619.
[16] 刘雅辉, 王秀萍, 张国新, 等. 棉花苗期耐盐生理指标的筛选及综合评价[J]. 中国农学通报, 2012, 28(6):73-78.
[17] 张宪政, 陈凤玉, 王荣富. 植物生理学实验技术[M]. 沈阳: 辽宁科学技术出版社, 1994: 66-69.
[18] Giannopolitis G N, Reis S K. Superoxide dismutase I. Occurrence in higher plants[J]. Plant Physiology, 1977, 59: 309-315.
[19] Aebi H. Catalase in vitro[J]. Methods in Enzymology, 1984, 105:121-126.
[20] Nakano Y, Asada K. Hydrogen peroxide is scavenged by ascorbate peroxidase in spinach chloroplasts[J]. Plant and Cell Physiology, 1981, 22: 867-880.
[21] Cakmak I, Marschner H. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves[J]. Plant Physiology, 1992, 98: 1222-1227.
[22] Elstner, E. F., Heupel, A.. Inhibition of nitrite formation from hydroxylammoniumchloride: a simple assay for superoxide dismutase[J]. Analytical Biochemistry, 1976, 70: 616–620.
[23]Brennan T, Frenkel C. Involvement of hydrogen peroxide in the regulation of senescence in pear[J]. Plant Physiology, 1977, 59: 411-416.
[24] Simon E W. Phospholipids and plant membrane permeability [J]. New Phytologist, 1974, 73(3): 377-420.
[25] 周志文. 葡萄种质资源的耐盐性鉴定及其生理基础的研究[D]. 山东农业大学, 2003.
[26] Asada K. The water-water cycle in chloroplasts：Scavenging of active oxygen and dissipation of excess photons[J]. Annual Review of Plant Physiology and Plant Molecular Biology, 1999, 50: 601-639.
[27] Shigeoka S, Ishikawa T, Tamoi M. Regulation and function of ascorbate peroxidase isoenzymes[J]. Journal of Experimental Botany, 2002, 53(372):1305-1319.
[28] Smirnoff N. The role of active oxygen in the response of plants to water deficit and desiccation[J]. New Phytologist, 1993, 125(1):27-58.
[29] 李会云, 郭修武. 盐胁迫对葡萄砧木叶片保护酶活性和丙二醛含量的影响[J]. 果树学报, 2008, 25(2): 240-243.
[30] 王艺陶, 周宇飞, 李丰先, 等. 基于主成分和SOM 聚类分析的高粱品种萌发期抗旱性鉴定与分类[J]. 作物学报, 2014, 40(1):110-121.
[31] 孙璐, 周宇飞, 汪澈, 等. 高粱品种萌发期耐盐性筛选与鉴定[J]. 中国农业科学, 2012, 45(9):1714-1722.