The paper aims to select the main indexes of the effects of NaCl stress on grapevine and provide theory basis for evaluation the salt resistance system of grapevines. With the annual of grapes ‘Tamina’ grown on the sand as the experimental material, six concentration of NaCl treatments were conducted, then the relative conductivity of grape root and leaf, the oxygen anion production rate, H2O2 content, antioxidant enzyme (SOD, CAT, POD, APX) activity of leaves, shoot length and total biomass of plants were analyzed. Results showed that the root and leaf relative conductivity increased gradually with the increase of NaCl concentration compared with the control, the super oxygen anion production rate and H2O2 content increased, SOD and CAT activity in the leaves presented a trend of rise-down, APX activity presented a trend of down-rise-down, while POD activity presented a downward trend; under high concentrations of NaCl treatment, the length of shoot and total biomass of the plants were reduced, higher oxygen anion production rate and H2O2 content in grape leaves might be related with the lower antioxidant enzyme activity. The principal component analysis indicated that the shoot length, CAT activity, H2O2 content and APX activity of leaves could be used to evaluate the effects of different concentration of NaCl stress on grapevine.