Abstract: Pear psylla (Psylla Chinensis) and spirea aphid (Aphis spiraeccla) are two kinds of main piercingsucking pests in pear orchard. We compared the toxicity of four bio-pesticides (matrine, pyrethrin, abamectin and nicotinic·matrine) against pear psylla and spirea aphid. The results showed that the four bio-pesticides had some inhibitory effect on spirea aphid and pear psylla, with the increase of concentration, the mortality increased, and the effect on spirea aphid was higher than that of pear psylla. The LC50 of pear psylla were matrine 129.84 mg/L, pyrethrin 144.7 mg/L, abamectin 90.74 mg/L and nicotinic·matrine 72.13 mg/L, that of spirea aphid were matrine 18.79 mg/L, pyrethrin17.79 mg/L, abamectin 0.2 mg/L and nicotinic · matrine 8.65 mg/L. The control effect on pear psylla ranged from 2.23% to 77.27% and the control effect on spirea aphid ranged from 43.2% to 93.69% at laboratory concentration. In conclusion, the four bio-pesticides showed good control effects on spirea aphid, and abamectin and nicotinic·matrine could be recommended as control agents on pear psylla.