Plasma Type and Temperature: Effects on the Non-parasitic Metamorphosis of Glochidia and Growth of Early Juvenile Mussel of Cristaria plicata

doi:10.11923/j.issn.2095-4050.cjas18030014

Abstract

Abstract: To improve the in vitro culture of Cristaria plicata glochidia, the in vitro cultivation technique was adopted to study the effects of blood serum and temperature on metamorphosis of glochidia, and the survive and growth of juvenile mussel at earlier stage were evaluated. The results showed that the metamorphosis rate of glochidia in bighead plasma was significantly higher than that of horse serum and bovine serum group (P< 0.01); the metamorphosis rate of glochidia cultured at 18℃ was significantly higher than that at 30℃ and 24℃ (P<0.05), the biological zero point of Cristaria plicata glochidia was 9.34℃ and the effective accumulated temperature was 102.84℃· d. After one month cultivation of juvenile mussel at earlier stage, the survival rate of juvenile mussel cultured in bighead plasma was the highest (P<0.05), and the shell length of juvenile mussel in the bighead plasma group was significantly higher than that of bovine serum group and horse serum group (P< 0.05). Under the three different temperature for cultivation, the lower the temperature, the higher the survival rate, and the difference was significant (P<0.05). Taken together, Cristaria plicata larva cultivated with bighead plasma under 18℃ has the highest metamorphosis rate, and the mussel larva has the highest survival rate.

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