Abstract:

The factors affecting the SRAP-PCR analysis of Asparagus officinalis, such as concentration of dNTPs, Taq DNA polymerase, Mg²⁺, primer and template DNA, were optimized by an orthogonal design. It is found that the optimal SRAP-PCR reaction conditions were a reaction system with a total volume of 20 μL, 0.2 mmol/L dNTPs 0.4 μL, 1 U Taq DNA polymerase 1.0 μL, 1.5 mmol/L Mg²⁺ 1.2 μL, upper and lower primers each 30 ng and each primer 0.5 μL, 60 ng template DNA 1.0 μL, 10×Buffer 2.0 μL, and sterile distilled water 13.4 μL.