Abstract:

Objective to study the isolation and characterization of the nitrite-oxidizing bacteria and identification by 16S rDNA. Methods one pure culture N3, which was enriched and isolated based on the procedures for nitrite oxidizer from sludge of a lake in Huzhou. Total DNA of pure culture was extracted and specific primers for the 16S rDNA of nitrite oxidizer were used for polymerase chain reaction (PCR). The amplified product was analyzed by agarose gel e1ectrophoresis and Sanger sequencing. Homology analysis was made by searching in Genbank with NCBI-Blast. Results the amplified fragment was 1441 bp for N3. 100% of N3 nucleotides were identica1 with the conserved fragment of Cellulosimicrobium cellulans (AY665978.1). Conclusion N3 phy1ogenetica1ly belonged to Cellulosimicrobium cellulans.