Abstract:

To study the homology and evolutionary relations among CHS (chalcone synthase) genes, CHS gene in Petunia hybrida, Vilm. was took as explants material, base distribution, amino acids composition, prediction of hydrophobicity or hydrophilicity, and multiple alignments with other 10 species were analyzed by the software and bioinformatics web site. The results showed that the mRNA single sequence contained 1170 bp base, the number of amino acids encoding protein was 389 and its relative molecular mass was 42580.02, Leucine achieved the highest content of 10.80%, hydrophobic maximum value was 2.038, hydrophobic minimum value was -2.208, and no obvious hydrophilic or hydrophobic area. The homology of multiple alignment of the amino acid sequence for CHS genes in 11 species achieved 80.55%. The results showed that the genes were in a stable state, encoded proteins were hydrophilic protein, and the evolutionary process was conservative. The conservative sequence information achieved in these CHS genes laid a foundation for other new CHS gene cloning.