Abstract:

This study aims to explore the screening dose of hygromycin suitable for Lycium barbarum, and lay a foundation for the study of gene function and genetic transformation of L. barbarum. In this study, the leaves of germless L. barbarum seedlings from Ningxia were used as explants, and four hygromycin concentration gradients were set to study the effect of hygromycin on the induction of callus and callus differentiation buds, to screen out the appropriate antibiotic dose for L. barbarum transformation body screening. The results showed that the increased concentration of hygromycin had a significant inhibitory effect on the induced callus and bud differentiation of L. barbarum. The higher the concentration of hygromycin, the lower the recovering rate of L. barbarum leaves was, and the browning rate of callus increased, and the leaves gradually became yellow, albino, and even died. Using hygromycin as a selective marker for resistance, the optimal concentration of hygromycin is 2 mg/L.

Key words: Lycium barbarum, hygromycin, resistance concentration, selected marker, genetic transformation