Resistance of Erysiphe necator to Tebuconazole in China

doi:10.11923/j.issn.2095-4050.cjas2022-0063

Abstract

Abstract:

To clarify the sensitivity of Erysiphe necator isolates to tebuconazole in China, quantitative real-time PCR (qPCR) was used to analyze the resistance of 134 E. necator isolates collected from 5 grape growing provinces, based on the detection of mutation A495T at locus 495 of the CYP51 gene of E. necator. The spore germination method was used to determine the susceptibility of the test isolates to tebuconazole, and the correlation between the results of the two methods was analyzed. The results showed that E. necator isolates had developed resistance to tebuconazole in China, while the resistance frequency was 35.07%, with differences in different regions. The EC₅₀ value of all isolates to tebuconazole determined by spore germination method ranged from 0.085 to 280.917 μg/mL and the average EC₅₀ value was 24.208 μg/mL. The ratio of the lowest and the highest EC₅₀ values for the isolates from different regions ranged from 2.5 to 3304, indicating that the sensitivity of the isolates from different regions to tebuconazole varied greatly. The results of tebuconazole sensitivity detection of the spore germination method and qPCR method were consistent and significantly correlated.

Key words: Erysiphe necator, tebuconazole, fungicide resistance, quantitative real-time PCR, spore germination

GAO Qi, LIU Mei, TAN Haiyun, WANG Qi, LI Xinghong, ZHANG Wei. Resistance of Erysiphe necator to Tebuconazole in China[J]. Journal of Agriculture, 2023, 13(6): 49-54.

Figures/Tables 4

References 32

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采集地点	菌株数/株	抗性菌株数/株	A495T频率/%
北京市密云区	4	4	100
北京市朝阳区	14	5	35.71
北京市平谷区	22	6	27.27
北京市通州区	26	5	19.23
北京市房山区	21	2	9.52
湖南省岳阳市	6	3	50
江苏省张家港市	15	6	40
江苏省昆山市	20	3	15
云南省元谋县	3	1	33.33
宁夏自治区银川市	3	2	66.66
总计	134	47	35.07

采集地点	菌株数/株	抗性菌株数/株	A495T频率/%
北京市密云区	4	4	100
北京市朝阳区	14	5	35.71
北京市平谷区	22	6	27.27
北京市通州区	26	5	19.23
北京市房山区	21	2	9.52
湖南省岳阳市	6	3	50
江苏省张家港市	15	6	40
江苏省昆山市	20	3	15
云南省元谋县	3	1	33.33
宁夏自治区银川市	3	2	66.66
总计	134	47	35.07

采集地点	菌株数/株	EC₅₀/(μg/mL)			敏感性指数
采集地点	菌株数/株	范围	差异倍数/倍	平均数±标准差	敏感性指数
北京市密云区	4	18.377~114.826	6.2	51.164±43.271	2.11
北京市朝阳区	14	3.343~49.603	14.8	21.396±13.361	0.88
北京市平谷区	22	4.358~43.424	9.9	19.247±11.937	0.79
北京市通州区	26	2.969~41.769	14.1	18.139±9.074	0.67
北京市房山区	21	2.580~59.048	22.9	16.549±13.573	0.75
湖南省岳阳市	6	4.851~28.642	5.9	17.471±9.383	0.72
江苏省张家港市	15	7.048~52.695	7.5	23.226±14.797	0.95
江苏省昆山市	20	4.559~70.519	15.5	31.123±14.797	1.29
云南省元谋县	3	7.438~18.314	2.5	13.996±5.774	0.58
宁夏自治区银川市	4	0.085~280.917	3304	195.198±180.968	8.06
总计	134	0.085~280.917	3304	24.208±28.039	1

采集地点	菌株数/株	EC₅₀/(μg/mL)			敏感性指数
采集地点	菌株数/株	范围	差异倍数/倍	平均数±标准差	敏感性指数
北京市密云区	4	18.377~114.826	6.2	51.164±43.271	2.11
北京市朝阳区	14	3.343~49.603	14.8	21.396±13.361	0.88
北京市平谷区	22	4.358~43.424	9.9	19.247±11.937	0.79
北京市通州区	26	2.969~41.769	14.1	18.139±9.074	0.67
北京市房山区	21	2.580~59.048	22.9	16.549±13.573	0.75
湖南省岳阳市	6	4.851~28.642	5.9	17.471±9.383	0.72
江苏省张家港市	15	7.048~52.695	7.5	23.226±14.797	0.95
江苏省昆山市	20	4.559~70.519	15.5	31.123±14.797	1.29
云南省元谋县	3	7.438~18.314	2.5	13.996±5.774	0.58
宁夏自治区银川市	4	0.085~280.917	3304	195.198±180.968	8.06
总计	134	0.085~280.917	3304	24.208±28.039	1