Abstract:

To explore the effects of different plant growth regulators, concentrations and treatment time on rooting of A. trifoliata cuttings, using the A. trifoliata introduced in Three Gorges Botanical Garden as test material, the orthogonal experiment design and the subordination function were used to comprehensively evaluate the rooting indexes. The results showed that the adventitious root formation of A. trifoliata belonged to the bark rooting, and the number of adventitious from node and internode accounted for 26.4% and 73.6% of the total number of adventitious roots, respectively. The effects of three factors on rooting were ranked as treatment time> plant growth regulator types> concentrations. Among the three treatment times (2, 4 and 6 h), the rooting effect of 4 h treatment had the best effectiveness; among the three plant growth regulators, IBA+ NAA (1:1) showed the best performance; and there were no significant difference among plant growth regulator concentrations (50, 150 and 250 mg/L). Among the 10 treatment combinations, 250 mg/L IBA+ 250 mg/L NAA for 2 h had the highest average subordinate function value and best rooting effect. This research provided scientific basis for propagation superior variety of A. trifoliata.

Key words: A. trifoliata, cutting propagation, plant growth regulators, orthogonal experiment design, rooting type, variance analysis, comprehensive evaluation