Current issue

• 2025 Vol.15 No.6 Published:20 June 2025

  Optimization and Application of Efficient Detection System for Extraction of Polysaccharides in Pumpkin SONG Hui, AN Xuejun, HUANG Yunping, ZHANG Xiangqin, LV Yan, QIAN Xinxin 2025, 15 (6):  1-9.  DOI: 10.11923/j.issn.2095-4050.cjas2025-0019 Abstract + ( 37 )   HTML ( 3 )   PDF ( 1903KB )   ( 6 ) To simplify the determination methods of pumpkin polysaccharide content in breeding process, the optimized experiments of phenol sulfuric acid method after hot water extraction of pumpkin polysaccharide were carried out. The detection wavelength and H2SO4 amount were determined by single factor experiment using 23S18 and other pumpkin flesh. The correlation of determination results between unpurified and purified polysaccharide content was clarified. The results showed that the wavelength of pumpkin polysaccharide determined by phenol-sulfuric acid method was 490nm, and the sample in reaction system included: 5% phenol: H2SO4 was 2:2:7. The regression equation was established as y=0.0061x+0.1476 (R2=0.9965) and the linear relationship was good when the absorbance was in the range of 0.084-3.684. There was a significant correlation between the unpurified and purified polysaccharide content. The polysaccharide contents of 53 pumpkin parents and combinations were determined and analyzed. Two parents (23S2 and 23S14) and two combinations (23FV26 and 23FV5) with high polysaccharide content of more than 100mg/g were selected for breeding. The efficient detection system for polysaccharides was simple to operate, wide in linear range, and the determination results are significantly related to the results of purified polysaccharide, which would improve the efficiency of pumpkin polysaccharide breeding.

  Study on Plant Type of Conventional Rice Varieties with High Light Efficiency in Yunnan Province LI Jianli, WANG Silai, ZOU Shu, ZHANG Haiqing, XIANG Zhangnen, PU Wei, ZHANG Yujiao, HUANG Guangfu 2025, 15 (6):  10-15.  DOI: 10.11923/j.issn.2095-4050.cjas2024-0059 Abstract + ( 29 )   HTML ( 2 )   PDF ( 1413KB )   ( 4 ) This study aimed to explore an ideal plant type model for high light efficiency rice cultivation in Yunnan Province, thereby promoting rice yields. The experiment utilized low light efficiency rice varieties, namely 'Chujing 27' (HP1), 'Taiwan Upland Rice' (HP2), 'AZUCENA' (HP3), and 'B3619C-7B-8-1-4' (HP4), as well as high light efficiency rice varieties, specifically 'Denong 205' (HP5), 'Dianjingyou 1' (HP6), 'Diantun' 502 (HP7), 'Yunda 107' (HP8), and 'Dianrui 449' (HP9), as experimental materials to investigate rice variety plant type patterns. A single-factor randomized block design was employed, with 9 rice varieties constituting the treatments and 4 replicates per treatment. Rice planting, water, and fertilizer management were conducted according to conventional rice cultivation methods, and relevant agronomic traits for high light efficiency were measured at maturity. The results indicated that the ideal plant type pattern of high light efficiency varieties in Yunnan Province exhibited the following characteristics: a plant height ranging from 90 to 110 cm; a panicle length of 22.0 to 25.3 cm; the number of secondary branches of 25.1 to 31.1; a flag leaf base angle of 10.5 to 17.1°, a flag leaf opening angle of 11.7 to 17.4°, a flag leaf length of 18.7 to 31.1 cm, and a flag leaf width of 1.4 to 1.8 cm. This experiment has elucidated the ideal plant type pattern for high light efficiency rice in Yunnan, thus providing a theoretical foundation for breeding such rice varieties.

  Study on Spatial Distribution and Sampling Technique of Clubroot Disease in Brassica rapa var. chinensis and Brassica rapa var. glabra Under Greenhouse Cultivation HONG Wenying, WU Yanjun, ZHU Xuyan, HUA Yonggang, WANG Qun, HE Linhai, SU Guijun, GAO Kai 2025, 15 (6):  16-22.  DOI: 10.11923/j.issn.2095-4050.cjas2024-0055 Abstract + ( 26 )   HTML ( 1 )   PDF ( 1311KB )   ( 3 ) Brassica rapa var. chinensis and Brassica rapa var. glabra were employed as experimental materials, with the sampling methods such as aggregation index, m*-m regression analysis and Taylor’s power law, and the spatial distribution pattern and characteristic of clubroot disease under greenhouse cultivation were analyzed, and the sampling techniques were studied to further improve the investigation and prediction ability of the disease. The result showed that the disease developed severe when the test area was closer to the center of the greenhouse, and relatively light when it was near the edge in the two crops. Test of aggregation index showed that the main trends of clubroot disease were fitted to uniform distribution under the condition of heavy occurrence, but aggregation distribution would also occur in the lower occurrence area. Regression analysis of m*-m and Taylor’s power law indicated that the individual colony was the basal component of the spatial distribution of clubroot disease and they attracted each other slightly. The distribution pattern of individual colony tended to be uniform distribution, and this trend was increased with the increasing disease grade of individual plant in the two crops. On the basis of the above analysis, the optimal theoretical sampling model and sequential sampling model of clubroot disease in the two crops were presented. This study results were helpful to improve efficiency in the investigation and sampling of clubroot disease, and it provided evidence for early-forecast and prevention decision.

  Comparative Analysis of Impact of Integrated Rice Farming Systems on Soil Ecological Environment of Paddy Fields LONG Li, HE Hui, HUANG Huang, CHEN Can, FU Zhiqiang 2025, 15 (6):  23-31.  DOI: 10.11923/j.issn.2095-4050.cjas2024-0040 Abstract + ( 27 )   HTML ( 3 )   PDF ( 1355KB )   ( 11 ) To study the influence of rice field integrative cultivation on soil, this article summarized the research progress on integrated rice farming models both domestically and internationally in recent years, and compared them with rice monoculture. The effects of different modes of rice field integrative cultivation on the ecological environment of paddy soil were analyzed from five aspects: soil fertility, soil microorganisms, soil enzyme activity, soil heavy metal and soil greenhouse gas emission. The results showed that compared to rice monoculture, integrated rice farming significantly improved soil quality, increased soil nutrients, optimized soil structure, promoted soil biodiversity, reduced heavy metal accumulation, and reduced greenhouse gas emissions. These findings provided important references for agricultural production and demonstrate the potential of integrated rice farming models in promoting sustainable agricultural development and ecological environment protection.

  Evaluation of Cultivated Land Ecological Security and Its Obstacle Factors in Hebei Province Based on DPSIR Model WANG Yanqiang, WANG Yue, CHENG Chi 2025, 15 (6):  32-41.  DOI: 10.11923/j.issn.2095-4050.cjas2024-0206 Abstract + ( 21 )   HTML ( 2 )   PDF ( 1570KB )   ( 3 ) This study aims to assess the ecological status of cultivated land in Hebei Province and identify associated issues, with the objective of providing recommendations to enhance the ecological security of cultivated land in the region. The methodology employed involved the systematic analysis of annual changes in key driving factors and primary obstacles. The evaluation index system of cultivated land ecological security in Hebei Province was established based on the Driving Force-Pressure-State-Impact-Response (DPSIR) model. This system was used to systematically assess the dynamic changes of cultivated land ecological security in the province during the period from 2011 to 2020. The analysis also utilized the obstacle degree model to identify and analyze the primary factors hindering the enhancement of the cultivated land ecological security index in Hebei Province. The results indicated that (1) the comprehensive level of cultivated land ecological security in Hebei Province from 2011 to 2020 increased from the ‘critical security’ level to the ‘safer’ level, manifesting a sustained positive trajectory; (2) the cultivated land ecological security of Hebei Province was divided into two distinct phases: 2011-2017 and 2017-2020. The security index exhibited a decline in the first stage due to the impacts of pesticides, chemical fertilizers, and agricultural films, while it increased in the second stage due to the transformation of agriculture to a higher level of green development; (3) the primary impediments from 2011 to 2020 had undergone a shift, transitioning from the ratio of effective irrigated area, proportion of primary industry, and the intensity of chemical fertilizer, pesticide, and agricultural film utilization per unit of cultivated land to the land reclamation rate, population density, urbanization level, and the proportion of primary industry. Consequently, a series of recommendations have been proposed to enhance the cultivated land ecological security. These recommendations encompass the development of green agriculture, augmented investment in cultivated land, population control measures, the refinement of the cultivated land protection system, and the promotion of public awareness regarding cultivated land protection.

  The Effects of Different Water Management Measures on Absorption and Accumulation of Cadmium by Rice in Moderately Cadmium Polluted Farmland SONG Xiaoqin, CHEN Guoan, CHEN Fuming, YE Zhengqian 2025, 15 (6):  42-49.  DOI: 10.11923/j.issn.2095-4050.cjas2024-0065 Abstract + ( 35 )   HTML ( 3 )   PDF ( 1307KB )   ( 6 ) The aim was to explore the application effects of different water management measures in cadmium (Cd) moderately polluted farmland and ensure food safety production. In this experiment, five water management measures (conventional irrigation, whole growth period flooding, moist irrigation, periodic moist irrigation and twice field drying at tillering-heading stage) were adopted to carry out the indoor pot experiments. The experiment measured the growth status of rice, the Cd content in different parts of rice at various growth stages (tillering stage, booting stage, filling stage and maturity stage), and calculated the Cd enrichment and transport coefficients as well as changes in soil pH and organic matter. The results showed that the moist irrigation treatment increased the 1000-grain weight of rice by 8.64%, and significantly reduced the Cd content in the roots, stems and leaves of rice at tillering stage. Compared with conventional irrigation treatment, moist irrigation could significantly reduce the Cd content in mature rice grains, with a decrease of 78.83% (P<0.05), followed by the whole growth period flooding treatment (with a decrease of 39.69%). In addition, the whole growth period flooding and moist irrigation treatments mainly reduced the Cd content in rice by inhibiting the migration of Cd from stems and leaves to grains. Based on rice production and actual situation, it was recommended to use moist irrigation as a water management measure for rice planting in moderately Cd-contaminated farmland.

  Screening and Application of Cellulose Degradation Bacteria: A Review ZHANG Xiaojie, CHEN Junling, WANG Shasha, LI Yanchun, LI Zhaowei, WANG Yixiang 2025, 15 (6):  50-56.  DOI: 10.11923/j.issn.2095-4050.cjas2024-0140 Abstract + ( 31 )   HTML ( 3 )   PDF ( 1120KB )   ( 3 ) To explore new approaches for the application of efficient cellulose-degrading microorganisms in agriculture, this study conducted a literature review and analysison the screening of cellulose degrading bacteria and the optimization of enzyme production in recent years, summarizing and analyzing four key aspects: strain types, enzyme production condition optimization, construction of composite microbial communities, and applications of these microbial communities in agriculture. The study outlines different screening strategies and advantages of various strains, analyzes the raw material types and strain specificity for optimizing cellulose-degrading enzyme production conditions, and discusses the necessity and significance of constructing composite microbial communities. Additionally, it provides a comprehensive overview of the applications of cellulose-degrading microorganisms (or communities) in three areas: biofertilizers, crop residue utilization, and bioenergy. The study identifies current limitations, such as the limited variety of cellulases produced by single strains and the need for optimized enzyme production conditions in composite microbial communities. To address these issues, the study proposes focusing on enhancing the screening of efficient cellulose-degrading microorganisms, utilizing molecular biology techniques to construct gene banks for cellulose-degrading microorganisms, and studying their degradation mechanisms. These efforts aim to improve the efficiency of screening for cellulose-degrading microorganisms, reduce the waste of agricultural resources, and promote rapid agricultural development and resource recycling.

  Investigation of Wild Cherry Varieties in Hanzhong LI Jiangyue, WU Puxia, QIU Feng, WANG Li, CHEN Yumin, BAI Wenzhao 2025, 15 (6):  57-64.  DOI: 10.11923/j.issn.2095-4050.cjas2024-0108 Abstract + ( 22 )   HTML ( 1 )   PDF ( 1737KB )   ( 2 ) Hanzhong city is located on the north slope of the Bashan Mountain, the southern slope of the Qinling Mountains, and in the demarcation zone of the north and south climate in China, it belongs to the north subtropical zone to the warm temperate zone, and is known as the "biological gene bank". There are abundant wild cherry variety resources in this area. Based on the survey and field investigation on the southern slope of Qin Mountains and the northern slope of Bashan (Guangwu Mountain), the results showed that there were 23 wild cherry varieties in this area, in the Qin Mountains and Bashan Mountains (600-1500 m), flowering time, petal color, fruit shape, taste, soluble solids content, the morphological diversity of seed shape, size and pollen grain shape was obvious. This investigation provides scientific basis for further exploring wild cherry variety resources, utilization and improvement of production varieties in Qinling-Bashan mountain area.

  Comparative Analysis of Agronomic Characters, Photosynthetic Parameters and Quality Characters of Celery in Autumn and Winter in Huai’an Area SUN Xu, CHEN Yuezhen, CHEN Chen, WANG Lixiang, WEI Yongju, SUN Yuehua, LI Yapeng, LIU Peizhuo, WANG Guanglong, XIONG Aisheng 2025, 15 (6):  65-70.  DOI: 10.11923/j.issn.2095-4050.cjas2024-0078 Abstract + ( 29 )   HTML ( 1 )   PDF ( 1802KB )   ( 5 ) In order to screen for Huai'an Medicinal Celery (HY) strains that are more suitable for cultivation in the Huai'an region and have excellent comprehensive quality, ‘HY-1’, ‘HY-2’, ‘HY-3’ and ‘HY-4’ were used as experimental materials to compare agronomic traits, photosynthetic characteristics and nutritional quality using significant difference analysis and correlation analysis. The results showed that ‘HY-3’ was dominant in the four lines except for leaflet width, plant height and spread degree. The most obvious advantages were the number of leaflet (172.67 per plant) and plant weight (256.60 g/per plant). Among the four lines, ‘HY-3’ had the highest net photosynthetic rate and water use efficiency, while ‘HY-4’ had the highest transpiration rate, stomatal conductance and intercellular CO2 concentration. Leaflet chlorophyll content (42.13 SPAD), apigenin (1.55 mg/g in leaflet, 0.88 mg/g in petiole) and luteolin (1.77 mg/g in leaflet, 0.28 mg/g in petiole) of ‘HY-3’ were the highest among the 4 lines, and lignin content was relatively high (12.82 mg/g in leaflet, 10.17 mg/g in petiole). The correlation analysis showed that the leaflet length and leaflet width were positively correlated, and the contents of apigenin and luteolin were positively correlated in the 4 ‘HY’ lines. In summary, the ‘HY-3’ has better agronomic traits, photosynthetic capacity, water utilization rate and nutritional quality, and has higher adaptability to stress and high yield potential.

  Effects of N P K Fertilizers Combined Application on Yield and Quality of Chinese Chive QIN Huawei, GAO Junjie, WANG Ting, HAN Long, LIU Zhongliang, CHEN Zhen, YAN Weiqiang, GU Duanyin 2025, 15 (6):  71-76.  DOI: 10.11923/j.issn.2095-4050.cjas2024-0092 Abstract + ( 26 )   HTML ( 2 )   PDF ( 1339KB )   ( 9 ) The effects of different nitrogen, phosphorus and potassium ratios on the yield and quality of Chinese chive were analyzed to provide a theoretical basis for scientific and rational fertilization. The ‘3414’ fertilizer efficiency design was adopted. Three factors of nitrogen (N), phosphorus (P) and potassium (K) were set up, and four levels of each factor were 0, 112.5, 225 and 337.5 kg/hm2, a total of 14 treatments. The tiller number, fresh weight per plant, yield and pyruvic acid were measured. By fitting the yield and nitrogen, phosphorus, and potassium fertilizer effect functions, the optimal application amount was determined. The results showed that the application of nitrogen, phosphorus, and potassium fertilizers increased the tillering number, fresh weight per plant, and yield of Chinese chives. The effects of the three fertilizers on yield was in the order of N>P>K. N1, P1 and K1 had the highest agricultural efficiency by 116.04, 73.22 and 61.75 kg/kg. N2, P2 and K2 had the highest contribution rates of fertilizers by 44.34%, 27.76% and 25.43%. However, excessive application of N, P and K fertilizers had the lowest agricultural efficiency and contribution rate. N, P and K fertilizers could significantly affect the content of pyruvic acid. In high fertility soil, the optimal fertilization rates for N, P and K are 186.32, 183.98 and 179.58 kg/hm2.

  Assessment and Prospects of Fluoride Reduction Measures in Brick Tea Production YIN Chunyan, ZHANG Li, YU Tingting, JIANG Ying, LIU Jie, ZHANG Ju 2025, 15 (6):  77-84.  DOI: 10.11923/j.issn.2095-4050.cjas2024-0073 Abstract + ( 21 )   HTML ( 1 )   PDF ( 1656KB )   ( 2 ) This paper aims to summarize and scientifically evaluate the effectiveness of existing fluoride reduction measures, providing validation and reference for further research on fluoride reduction strategies. This paper employed a combination of literature analysis, field investigations, and experimental validation to systematically assess the feasibility and effectiveness of various fluoride reduction measures at different stages, including tea tree cultivation, tea leaf harvesting, raw tea production, brick tea production, and tea consumption. Through systematic analysis and experimental validation, it was found that selecting low-fluoride tea tree varieties during cultivation and controlling the harvest time during tea leaf collection effectively reduced the fluoride content at the source. The water washing process during brick tea production was able to lower the fluoride content in the tea leaves to some extent. Additionally, adopting scientifically recommended tea-drinking practices reduced the fluoride intake from brick tea. Calcium phosphate and hydroxyapatite were identified as safe and effective fluoride adsorption materials. However, fluoride reduction effects were not significant when improving tea garden environments, using water blanching techniques, or adding fluoride-reducing materials during the pile fermentation process. Research on fluoride reduction measures in brick tea needs to further enhance the scientific evaluation process, improve the feasibility of practical applications, and fully consider the impact of these measures on the quality and safety of brick tea.

  Research Progress on Nutritional Value and Development and Utilization of Burdock Root DU Furong, LI Ke, ZHAO Mingming, HU Xinyan, LI Xiaoshan, SU Zaixing, HUANG Zhongqin, CHEN Xiaoguang 2025, 15 (6):  85-91.  DOI: 10.11923/j.issn.2095-4050.cjas2024-0083 Abstract + ( 31 )   HTML ( 1 )   PDF ( 1225KB )   ( 4 ) Burdock root is rich in numerous functional active substances and exhibits functions such as lowering blood glucose, exerting antibacterial, anti-inflammatory, and antioxidant activities, regulating lipid metabolism, and modulating the intestinal flora. It is renowned as the ‘King of Vegetables’ and ‘Oriental Ginseng’. This study places emphasis on the types and physiological functions of active substances such as polysaccharides, polyphenols, dietary fibers, amino acids, fatty acids, volatile oils, lignans, phytosterols, and pentacyclic triterpenes in burdock root. It also summarizes the current status of the development and utilization of primary and advanced processing products of burdock root. On this basis, the development of the burdock root industry is prospected, with the aim of providing a reference for the application of burdock root in fields such as food, medicine, and the chemical industry, as well as for its high-quality industrialization.

  Analysis of Coupling Status Between Precipitation and Water Use During Maize Growing Season in Liaoning Province ZHANG Li 2025, 15 (6):  92-100.  DOI: 10.11923/j.issn.2095-4050.cjas2024-0159 Abstract + ( 28 )   HTML ( 1 )   PDF ( 1850KB )   ( 4 ) To study the water balance during the maize growing season in Liaoning Province based on the meteorological data and crop coefficients in Liaoning Province from 1963 to 2022, the SIMETAW model and climate diagnostic analysis methods were used to analyze the precipitation, water demand patterns, and water satisfaction status during the maize growing season in Liaoning Province. The impact of regional climate change on maize water demand was revealed, and the spatio-temporal evolution of effective precipitation and water demand during the maize growing season in Liaoning Province was studied. The research results showed that the effective precipitation during the whole growth period reached 481.6 mm. Among them, it was 347.4 mm in the west, 514.9 mm in the central and southern parts, and 636.8 mm in the east. The average water demand during the whole growth period of maize was 521 mm, showing no significant downward trend. The average coupling degree between precipitation and water demand during the whole growth period of maize was 0.715, that is, precipitation met 71.5% of the water demand, and the average water shortage was 28.5%. The guarantee rate of the coupling degree λ > 0.8 in the western region was only 28.3%. The maximum value of the coupling degree appeared in the east of Liaoning Province, followed by the central and southern parts of Liaoning Province, and the minimum value appeared in the west of Liaoning Province. The highest value of the coupling degree appeared during the flowering and pollination period, followed by the trumpet stage, and the lowest value appeared in the early growth stage and the maturity stage. In recent years, the coupling degree between precipitation and water demand in the early growth stage of maize has shown a significant upward trend. The coupling degree between precipitation and water demand in the early growth stage of maize is relatively low. Therefore, it is necessary to pay attention to the occurrence of drought, especially in the western part of Liaoning Province where water resources are scarce.