关键词: 大花黄牡丹, 大花黄牡丹, 变异材料, 光合特性, 拉萨

Abstract: To select the suitable method for total RNA extraction from the style of Rosa rugosa, and provide foundation for related genetic engineering research, total RNA was extracted with three methods, including modified CTAB, Trizol and EASY spin Plus Plant RNA Kit. And the purity, concentration and completeness were compared by nucleic acid protein detector and gel electrophoresis. The effectiveness of gene amplification of reverse transcription product was checked by RT- PCR reaction. The results showed that the purity, concentration and completeness of total RNA extracted by modified CTAB and EASY spin Plus Plant RNA Kit were higher than that by Trizol. But the bands of total RNA extracted by modified CTAB had serious degradation and the Kit was clear. And the 28S was two times as bright as 18S. The reverse transcription of cDNA obtained for RT-PCR could clearly amplify specificity band. These results demonstrate that the RNA isolated by EASY spin Plus Plant RNA Kit is of sufficient quality and can satisfy subsequent molecular study for Rosa rugosa.