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农学学报 ›› 2023, Vol. 13 ›› Issue (6): 25-31.doi: 10.11923/j.issn.2095-4050.cjas2022-0056

• 农艺科学 生理生化 • 上一篇    下一篇

水氮互作对花针期花生叶片光合性能及荧光特性的影响

修俊杰(), 刘学良(), 韩彦龙, 谢志强   

  1. 铁岭市农业科学院,辽宁铁岭 112000
  • 收稿日期:2022-05-07 修回日期:2022-08-18 出版日期:2023-06-20 发布日期:2023-06-15
  • 通讯作者: 刘学良,男,1979年出生,河北承德人,研究员,本科,主要从事花生育种及栽培研究。通信地址:112000 辽宁省铁岭市银州区柴河街南段238号 铁岭市农业科学院,E-mail:lxliang0@yeah.net
  • 作者简介:

    修俊杰,女,1980年出生,辽宁铁岭人,高级农艺师,硕士,主要从事花生育种及栽培研究。通信地址:112000 辽宁省铁岭市银州区柴河街南段238号 铁岭市农业科学院,E-mail:

  • 基金资助:
    辽宁省科学技术厅农业攻关及产业化项目“优质; 高产花生增产提效关键技术研究与集成应用”(2020JH2/10200010)

Effect of Nitrogen Application on Photosynthesis and Fluorescent Characteristics of Peanut Under Drought-stress in Flowering-Pegging Stage

XIU Junjie(), LIU Xueliang(), HAN Yanlong, XIE Zhiqiang   

  1. Tieling Academy of Agricultural Sciences, Tieling 112000, Liaoning, China
  • Received:2022-05-07 Revised:2022-08-18 Online:2023-06-20 Published:2023-06-15

摘要:

于铁岭市花生示范基地防雨棚中进行,设正常灌水(田间持水量75%左右)和花针期干旱处理(土壤含水量自然减少持续8 d,植株萎蔫后复水至正常水处理),副处理设0、90、180 kg/hm2 3个氮水平,研究花针期氮对干旱条件下花生叶片光合性能与荧光特性的影响,以期为花生花针期遇干旱时合理施用氮肥提供理论依据。结果表明,干旱胁迫显著降低了土壤相对含水量、净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)和胞间CO2浓度(Ci),提高了叶绿素含量。干旱处理下PnTrGsCi、叶绿素含量均以90 kg/hm2施氮水平最高。干旱处理下初始荧光(F0)显著升高,并随施氮量的增加而增大;最大光化学效率(Fv/Fm)、量子效率(ΦPSⅡ)、电子传递速率(ETR)和光化学猝灭系数(qP)都明显降低,但干旱胁迫使非光化学猝灭系数(NPQ)增大。干旱处理条件下Fv/Fm、ΦPSETRqP均以90 kg/hm2施氮水平最高。不施(0 kg/hm2)或过量(180 kg/hm2)施氮肥均不利于花生叶片光合作用。因此,花针期干旱胁迫条件下以90 kg/hm2较适宜提高花生叶片的光合作用和荧光特性,最终获得高产。

关键词: 花针期, 花生, 干旱, 氮素, 光合作用, 荧光特性

Abstract:

The study was carried out in the Experimental Station of Tieling, a normal irrigation (soil water content was about 75%) and a short-term drought-stress (soil water content was naturally reduced by 8 days, and re-watered to normal after wilting) at flowering-pegging stage of peanut were set up, with three nitrogen levels (0, 90, 180 kg/hm2), to explore the effect of nitrogen application on photosynthesis and fluorescence characteristics of peanut under drought, thus providing reference for nitrogen application at peanut flowering-pegging stage. The results showed that soil water content, net photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs) and intercellular CO2 concentration (Ci) were significantly reduced, but chlorophyll content of peanut was increased under drought-stress. Under drought-stress, Pn, Tr, Gs, Ci and chlorophyll content of 90 kg/hm2 treatment were the best among the three levels of nitrogen application. Under drought-stress, the initial fluorescence of leaf (F0) was significantly increased with the increase of nitrogen; the maximum photochemical efficiency of photosystemⅡ(Fv/Fm), the quantum efficiency of electron transport (ФPSⅡ), the electron transport rate (ETR) and the photochemical quenching co-efficient (qP) significantly declined, but the non-photochemical quenching co-efficient (NPQ) was significantly increased. Under drought-stress, Fv/Fm, ФPSⅡ, ETP and qP reached the highest level at 90 kg/hm2 nitrogen application level. No application (0 kg/hm2) or excessive application (180 kg/hm2) of nitrogen fertilizer was not conducive to photosynthesis of peanut leaves. All results suggest that 90 kg/hm2 is the optimal nitrogen level to improve photosynthesis and fluorescence characteristics of peanut, and achieve high yield.

Key words: flowering-pegging stage, peanut, drought, nitrogen, photosynthesis, fluorescent characteristics