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农学学报

农学学报 ›› 2023, Vol. 13 ›› Issue (6): 32-38.doi: 10.11923/j.issn.2095-4050.cjas2022-0117

• 植物保护 • 上一篇    下一篇

甘薯茎腐病田间症状识别与快速镜检诊断研究

张莉丽1(), 王丽2, 谢关林2, 余山红3()   

  1. 1 浙江省杭州市农业技术推广中心，杭州 310021
    2 浙江大学生物技术研究所，杭州 310058
    3 浙江省台州市农业科学研究院，浙江台州 317000
  • 收稿日期:2022-08-25 修回日期:2023-02-10 出版日期:2023-06-20 发布日期:2023-06-15
  • 通讯作者: 余山红，男，1985年出生，湖北黄冈人，高级农艺师，硕士，从事农作物病虫害防治技术研究。通信地址：317000 浙江省台州市临海七一河路57号，Tel：0576-85196587，E-mail：glxie@zju.edu.cn，277298512@qq.com。
  • 作者简介:

    张莉丽，女，1977年出生，浙江上虞人，高级农艺师，硕士，主要从事植物保护与植物检疫工作。通信地址：310021 杭州市航海路768号 杭州农业大楼，Tel：0571-86781298，E-mail：。

  • 基金资助:
    浙江省重点研发项目“甘薯茎腐病关键防控核心技术研发”(2017C02002); 杭州市重点科技发展计划项目“甘薯茎腐病发病规律与防控技术研究及应用”(20172015A09); 浙江省基础公益研究计划科技计划“水稻白叶枯病再爆发原因及防控技术研究”(LGN19C140001)

Diagnosis of Bacterial Stem and Root Rot of Sweet Potato and Rapid Inspection of the Causal Pathogen by Microscope

ZHANG Lili1(), WANG Li2, XIE Guanlin2, YU Shanhong3()   

  1. 1 Hangzhou Agricultural Technology Extension Center, Hangzhou 310021, Zhejiang, China
    2 Institute of Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China
    3 Taizhou Academy of Agricultural Sciences, Taizhou 317000, Zhejiang, China
  • Received:2022-08-25 Revised:2023-02-10 Online:2023-06-20 Published:2023-06-15

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摘要：

为寻找一种适应基层植物检疫站应用的甘薯茎腐病产地检疫方法，以有效控制其传播与蔓延。通过多年田间发病全程观察及对甘薯茎腐病306份茎部和270份其他部位样本的显微镜检查并与传统和分子生物学方法比较和验证。研究表明甘薯茎腐病症状识别加显微镜喷菌现象观察能准确区分甘薯细菌性茎腐病与其他病原引起的甘薯茎基部腐烂病。明确了甘薯茎腐病初发病期的茎基水渍状斑始现时是镜检最佳和最有效时期，茎部是显微镜镜检的最佳部位。初期甘薯茎腐病症状识别加显微镜镜检喷菌现象的方法与传统和分子生物学方法匹配的准确率在98%~100%。这一方法不但准确、快速、简便、直观，而且适于国内基层植检站应用。

关键词: 甘薯, 茎腐病, Dickeya dadantii, 症状识别, 显微镜, 喷菌现象

Abstract:

To find out an inspection method suitable for primary plant quarantine station in origin area of bacterial stem and root rot of sweet potato caused by Dickeya dadantii, so that the disease can be effectively managed by limited dissemination and spread. The experiments were conducted by several years of field survey of the symptoms development and microscopic observation for the bacterial streaming of 306 stems and 270 other parts samples, and the results were compared and confirmed by traditional and molecular methods. The results showed that the symptom discriminating and microscopic observation for the bacterial streaming can accurately differentiate bacterial stem and root rot disease of sweet potato from other diseases caused by other pathogens. It was the best and effective time for microscopic observation of the bacterial streaming when the water-soaking symptom appeared at base of sweet potato plant in initial stage of the disease. Stem of sweet potato plant was the best position for microscopic observation of the bacterial streaming. The accuracy of initial symptom discriminating and microscopic observation for the bacterial streaming of the disease was more than 98% matched with traditional and molecular tests. It is an accurate, rapid, simple, visible, and suitable inspection method of bacterial stem and root rot disease of sweet potato for extension in primary plant quarantine stations in our country.

Key words: sweet potato, bacterial stem and root rot, Dickeya dadantii, symptom diagnosis, microscope, bacterial streaming