欢迎访问《农学学报》，
农学学报

农学学报 ›› 2011, Vol. 1 ›› Issue (7): 51-55.

所属专题： 水产渔业

• 畜牧 兽医 水产 • 上一篇    下一篇

饥饿胁迫对饲喂槐属植物黄酮的罗非鱼肝胰脏抗氧化能力的影响

刘淑兰 翟少伟 贾景涛   

  • 收稿日期:2011-06-28 修回日期:2011-07-15 出版日期:2011-09-26 发布日期:2011-09-26
  • 基金资助:

    福建省教育厅科技项目;集美大学优秀青年骨干教师基金

The Effects of Starvation Stress on the Antioxidant Parameter of Tilapia (Oreochromis spp.) fed with Sophora Flavonoids

  • Received:2011-06-28 Revised:2011-07-15 Online:2011-09-26 Published:2011-09-26

PDF (PC)

1585

摘要：

为了研究饥饿胁迫对饲喂槐属植物黄酮的罗非鱼肝胰脏抗氧化能力的影响，将400尾罗非鱼（平均体重为(11.52±2.85) g/尾）随机分为5个处理，每个处理4个重复。试验饲料SF添加量分别为0、200、400、800、1600 mg/kg，试验鱼正常饲喂7周后饥饿胁迫15天，测定饥饿胁迫第1天、第8天和第15天罗非鱼肝胰脏的丙二醛（MDA）含量、总抗氧化能力（T-AOC）以及超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性。结果表明：饲料中添加200~1600 mg/kg的SF可显著降低饥饿胁迫试验期末罗非鱼肝胰脏MDA含量和CAT活性（P＜0.01），显著提高应激时T-AOC水平（P＜0.01）和SOD活性（P＜0.05）；随SF添加水平的升高，罗非鱼肝胰脏抗氧化能力升高，1600 mg/kg SF添加组最佳。表明，SF可提高饥饿胁迫状态下罗非鱼肝胰脏的抗氧化能力。

关键词: 金边阔叶麦冬, 金边阔叶麦冬, 外植体, 激素配比, 组织培养

Abstract:

In order to study the effects of starvation stress on the antioxidant ability of tilapia that had been fed with Sophora flavonoids (SF), 400 fish were divided into 5 groups with 4 replicates in each group. Tilapia with average weight of (11.52±2.85) g per tail, were exposed to dietary SF at concentrations of 0, 200, 400, 800 and 1600 mg/kg for 7 weeks, then were starved for 1 d, 8 d, 15 d to measure the content of malonaldehyde (MDA), the total antioxidation capacity (T-AOC), and the activity of superoxide dismutase (SOD) and catalase (CAT) of the tilapia’s hepatopancreas. The results showed that 200 to 1600 mg/kg dietary SF significantly decreased the content of the MDA and the activity of CAT of the experimental groups (P＜0.01), and significantly increased the level of the T-AOC (P＜0.01) and the activity of the SOD (P＜0.05)of the experimental group. The antioxidant ability of tilapia increased with the increase of the level of dietary SF, and the group fed with 1600 mg/kg SF was the best. The results indicated that the ability of the oxidation resistance under starvation stress could be enhanced by adding SF in feed of tilapia.