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农学学报

农学学报 ›› 2013, Vol. 3 ›› Issue (2): 12-16.

所属专题： 马铃薯

• 农艺科学 作物遗传育种 生理生化 • 上一篇    下一篇

覆盖方式对马铃薯叶片氮代谢的影响

鄢铮   

  • 收稿日期:2012-11-13 修回日期:2012-12-04 出版日期:2013-02-20 发布日期:2013-02-20

Effects of Covering Methods on the Nitrogen Metabolism of Potato Leaves

  • Received:2012-11-13 Revised:2012-12-04 Online:2013-02-20 Published:2013-02-20

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摘要：

在大田条件下，以‘荷兰7号’马铃薯为材料，研究覆盖方式对其叶片氮代谢的影响，并探讨叶片全氮、可溶性蛋白含量与氮代谢相关酶活性之间的相关性。结果表明，从块茎形成期到淀粉积累期，不同处理的马铃薯叶片全氮、可溶性蛋白含量和硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、谷氨酸脱氢酶(GDH)活性均呈直线下降趋势，且6种覆盖处理均高于对照，差异达到显著或极显著水平。其中，以“草+白”处理值最高。相关性分析表明，各处理的马铃薯叶片全氮和可溶性蛋白含量与NR、GS和GDH活性之间呈显著或极显著正相关；各处理GS活性与GDH、NR活性呈显著或极显著正相关，NR活性与GDH活性呈显著或极显著正相关。

关键词: X射线荧光光谱法, X射线荧光光谱法, 镉, 原料公干鱼, 快速检测

Abstract:

Using transparent，black plastic film and grass as cover on potato(variety ‘Netherlands No. 7’ ) field，we compared the effects of different covering methods on the nitrogen metabolism of potato leaves，and we probed the interrelationships between the contents of the nitrogen and the soluble protein，and the activity of the nitrogen metabolism related enzyme. The results showed that，the contents of the nitrogen and the soluble protein，and the activity of nitrate reductase，glutamine synthase and glutamate dehydrogenase，all kept declining from tuber initiation stage to starch accumulation stage. And the indicators of potato covering different combinations were all higher than control，and their differences reached significant or extremely significant level，among which "straw and transparent plastic film" covering treatment were the highest. According to relevance analysis，in each treatment，the contents of the nitrogen and the soluble protein of potato leaves all had an significant or extremely significant positive correlation with the activity of NR，GS and GDH；And the activity of GS all had an significant or extremely significant positive correlation with and the activity of NR and GDH；And the activity of NR all had an significant or extremely significant positive correlation with and the activity of GDH.