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农学学报

农学学报 ›› 2015, Vol. 5 ›› Issue (5): 25-28.doi: 10.11923/j.issn.2095-4050.2014-xb0289

• 农艺科学 生理生化 • 上一篇    下一篇

高效液相色谱法同时检测虫草制品中腺苷和虫草素含量的研究

黄丽俊,李利东,宓晓黎   

  1. 江苏省微生物研究所有限责任公司 江苏省菩德食品安全检测技术有限公司,江苏省微生物研究所有限责任公司,江苏省微生物研究所有限责任公司
  • 收稿日期:2014-03-31 修回日期:2014-03-31 接受日期:2014-10-14 出版日期:2015-06-02 发布日期:2015-06-02
  • 通讯作者: 黄丽俊 E-mail:jspdhuang
  • 基金资助:
    无锡市社会发展科技支撑计划“构建食品中非法添加物快速检测关键技术集成及应用”(CSE01N1205)

Determination of adenosine and cordycepin in cordyceps preparation by HPLC

  • Received:2014-03-31 Revised:2014-03-31 Accepted:2014-10-14 Online:2015-06-02 Published:2015-06-02

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摘要： 对已有方法和规范中样品前处理方法和色谱条件进行了优化和对比研究，建立检测虫草制品中腺苷和虫草素含量的反相高效液相色谱方法。样品经水超声提取40 min，离心过滤后进行分析。采用C18色谱柱(250 mm×4.6 mm，5 μm)，以水-甲醇(85:15，V/V)为流动相，流速为1.0 mL/min，检测波长为260 nm。本方法检测限为0.2 μg/mL，定量限为1.0 μg/mL。以所建立的方法对虫草制品进行分析，样品加标回收率在94%~103%，相对标准偏差均小于3%。该检测方法简便、准确，并能同时测定腺苷和虫草素。

关键词: 机械化, 机械化, 收获, 甜荞品种, 植株性状

Abstract: To establish the quantitative analysis method for adenosine and cordycepin in Cordyceps sinensis by HPLC. The sample was extracted with pure water under ultrasonic for 40 min. After centrifugation and filtration, the extract was analyzed by HPLC with C18 column at 1.0 mL/min and detected at 260 nm wavelength. The mobile phase was composed of pure water and methanol(85:15, V/V) and the column temperature was 30℃ . This method showed the LOD was 0.2 μg/mL, LOQ was 1.0 μg/mL. The average recoveries of adenosine and cordycepin were 94%- 103% and RSD bellow 3% . The method had high sensitivity, selectivity and simple operation, and can be used for testing the adenosine and cordycepin.