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农学学报

农学学报 ›› 2021, Vol. 11 ›› Issue (1): 11-16.doi: 10.11923/j.issn.2095-4050.cjas20190900197

所属专题： 生物技术； 水稻

• 农艺科学/生理生化 • 上一篇    下一篇

利用HRM功能标记检测黑龙江省水稻抗稻瘟病基因Pita和pik的基因型分布

郭震华1,2(), 黄翠红2, 罗文龙2, 黄晓群1, 王瑞英1, 陈志强2, 关世武1, 张淑华1, 蔡丽君3   

  1. 1黑龙江省农业科学院水稻研究所,黑龙江佳木斯 154026
    2国家植物航天育种工程技术研究中心,广州 510642
    3黑龙江省农业科学院佳木斯分院,黑龙江佳木斯 154026
  • 收稿日期:2019-09-20 修回日期:2019-11-15 出版日期:2021-01-20 发布日期:2021-02-05
  • 作者简介:郭震华,男,1985年出生,内蒙古呼伦贝尔人,助理研究员,博士,主要从事水稻分子育种方面的研究。通信地址:154026 黑龙江省佳木斯市东风区省水稻研究所,Tel:0454-8841387,E-mail: hljsdsgzh@163.com。
  • 基金资助:
    国家重点研发计划“寒地早粳稻分子设计育种”(2016YFD0101801);黑龙江省农科院重大科研成果激励项目“Pita等抗稻瘟病基因在寒地稻区的分布研究”(2018KYJL001)

Detection of the Rice Blast Resistance Genes Pita and pik in Heilongjiang: HRM Functional Markers

Guo Zhenhua1,2(), Huang Cuihong2, Luo Wenlong2, Huang Xiaoqun1, Wang Ruiying1, Chen Zhiqiang2, Guan Shiwu1, Zhang Shuhua1, Cai Lijun3   

  1. 1Rice Research Institute of Heilongjiang Academy of Agricultural Sciences, Jiamusi 154026, Heilongjiang, China
    2National Engineering Research Centre of Plant Space Breeding, Guangzhou 510642, Guangdong, China
    3Jiamusi Branches of Heilongjiang Academy of Agricultural Sciences, Jiamusi 154026, Heilongjiang, China
  • Received:2019-09-20 Revised:2019-11-15 Online:2021-01-20 Published:2021-02-05

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摘要：

为明确Pita及pik基因在黑龙江省水稻种质资源中的分布情况,本研究以67份黑龙江省主栽品种及优异种质资源作为试验材料,通过HRM技术,利用Pita及pik的HRM功能型分子标记Pita-G/T、Pik2-C/T,对67份材料进行基因分型。结果表明,67份种质中,含纯合Pita有27份,占参试种质的40.3%;纯合pik有22份,占参试种质的32.9%,同时含有两基因的为12份,比例为17.9%。综上,Pita-G/T及Pik2-C/T 标记可以高效准确地对黑龙江水稻资源进行基因分型,同时,通过基因分型,明确了Pita及pik在黑龙江省的分布情况,为抗稻瘟病育种提供了理论基础。

关键词: 水稻, 高分辨率熔解曲线, Pita基因, pik基因, 基因分型

Abstract:

To clarify the distribution of Pita gene and pik gene in rice germplasm resources of Heilongjiang, 67 major varieties and excellent germplasm resources were used as materials with HRM (high resolution melting) technology. 67 materials were used for genotyping analysis by Pita-G/T and Pik2-C/T of Pita and pik HRM-based functional markers. The results showed that, among the 67 germplasm resources, 27 germplasm resources contained the homozygous gene Pita (40.3% of the total number), 22 germplasm resources contained the homozygous gene pik (32.9% of the total number) and 12 germplasm resources contained both of the two homozygous genes (17.9% of the total number). Based on the results, the functional markers Pita-G/T and Pik2-C/T could be efficiently and accurately used in rice genotyping in Heilongjiang, meanwhile, through genotyping the materials in this research, we clarified the distribution of those two genes in Heilongjiang, which provided a theoretical basis for rice blast resistance breeding.

Key words: Rice, High Resolution Melting (HRM), Pita Gene, pik Gene, Genotyping

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