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农学学报 ›› 2021, Vol. 11 ›› Issue (5): 74-82.doi: 10.11923/j.issn.2095-4050.cjas2020-0059

所属专题: 生物技术

• 农业工程/农业机械/生物技术/食品科学 • 上一篇    下一篇

谷子MRP蛋白家族序列特征、分子进化及表达模式分析

高豪(), 满夏夏, 孙朝霞, 韩渊怀, 李红英, 侯思宇, 郭数进()   

  1. 山西农业大学农学院,山西太谷 030801
  • 收稿日期:2020-04-09 修回日期:2020-06-28 出版日期:2021-05-20 发布日期:2021-06-29
  • 通讯作者: 郭数进 E-mail:haichongqishi@163.com;sxndgsj@163.com
  • 作者简介:高豪,男,1992年出生,四川三台人,在读硕士,研究方向:种质创新与遗传育种研究。通信地址:030801 山西省晋中市太谷县山西农业大学农学院,Tel:0354-6288344,E-mail: haichongqishi@163.com
  • 基金资助:
    国家重点研发计划“杂粮作物品质性状形成分子机理及调控途径”(2018YFD1000705-2);山西省高等学校科学研究优秀成果培育项目“小米特色功能营养成分形成机制”(2019KJ020);山西省重点研发项目“谷子杂种优势利用及基于全基因组SNP的技术开发”(201703D211008);山西农谷项目“山西省主要功能作物产业技术研究与示范-功能作物种质创新研究”(SXNGJSKYZX201702);山西省粮食局攻关项目“特色品质谷子品种筛选”(201803);山西省重点研发计划项目“高产富硒大豆新品系选育”(201803D221008-2);山西农业大学博士科研启动项目“山西不同生态型大豆抗旱应答机制的研究”(2015ZZ07);山西省研究生优秀创新项目“谷子叶酸富集性状全基因组关联分析”(2020SY207)

MRP Protein Family of Foxtail Millet: Analysis of Sequence Characteristics, Molecular Evolution and Expression Pattern

Gao Hao(), Man Xiaxia, Sun Zhaoxia, Han Yuanhuai, Li Hongying, Hou Siyu, Guo Shujin()   

  1. College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi, China
  • Received:2020-04-09 Revised:2020-06-28 Online:2021-05-20 Published:2021-06-29
  • Contact: Guo Shujin E-mail:haichongqishi@163.com;sxndgsj@163.com

摘要:

本研究旨在深入了解谷子叶酸转运家族成员的基因结构和表达模式,为谷子体内叶酸转运分子机制的研究奠定基础。使用Phytozome、Clustal X、MEGA7.0等在线工具和软件,对谷子MRP家族成员进行生物信息学分析;基于谷子谷穗有参转录组测序数据,分析谷子MRP家族成员表达模式。结果显示,谷子MRP蛋白家族成员共21个,在7号染色体分布最多,有8个基因;17个谷子MRP蛋白偏碱性和4个偏酸性,根据疏水值判断其均为亲水性蛋白;成员SiMRP7SiMRP12基因的表达量与谷子组织中的总叶酸含量表现出协同降低的趋势,推测这两个蛋白可能对谷子叶酸的积累起到调控作用。本研究有助于进一步鉴定叶酸转运相关蛋白,为后续研究叶酸代谢途径及谷子基因挖掘提供了理论基础。

关键词: 谷子, MRP蛋白家族, 谷子叶酸, 生物信息学分析, 表达模式

Abstract:

The aims are to study the gene structure and expression patterns of folate transport family members, and establish the foundation for exploring the molecular mechanism of folate transport in foxtail millet. Bioinformatics analysis of MRP family members was carried out with Phytozome, Clustal X, MEGA7.0 and other online tools and software. Based on the transcriptome sequencing data of foxtail millet, the expression patterns of foxtail millet MRP family members were analyzed. The results show that there are 21 foxtail millet MRP protein family members, most of which are distributed on chromosome 7, with 8 genes; 17 MRP proteins are alkaline and 4 proteins are acidic, and they are all hydrophilic proteins according to the hydrophobic value. The expression of SiMRP7 and SiMRP12 genes and the total folate content in foxtail millet tissue have a synergistic decrease trend, and these two proteins might play a regulatory role in the accumulation in foxtail millet. This study further identifies folate transport-related proteins, and could provide a theoretical basis for future research on folate metabolism pathway and gene mining.

Key words: Foxtail Millet, MRP Protein Family, Folate of Foxtail Millet, Bioinformatics Analysis, Expression Pattern

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