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农学学报 ›› 2025, Vol. 15 ›› Issue (2): 56-63.doi: 10.11923/j.issn.2095-4050.cjas2024-0004

• 林学 园艺 园林 食用菌 • 上一篇    下一篇

无花果组培快繁技术研究进展

冯帅帅1(), 乔峰1, 贾明2(), 魏娟2, 姜婷婷1, 黄爱玲1, 金志博3, 央宗1, 李爱花1, 韩葳1, 李全希1   

  1. 1 淄博市农业科学研究院,山东淄博 255033
    2 山东省林业科学研究院,济南 250000
    3 山东新坐标农业开发有限公司,山东淄博 256400
  • 收稿日期:2024-01-12 修回日期:2024-06-26 出版日期:2025-02-20 发布日期:2025-02-18
  • 通讯作者:
    贾明,女,1982年出生,山东泰安人,正高级工程师,博士,主要从事特色浆果良种选育、高效生产关键技术、天然产物/生化成分分析等科研与推广工作。通信地址:250014 山东省济南市历下区文化东路42号,Tel:0531-88557772,E-mail:
  • 作者简介:

    冯帅帅,男,1991年出生,山东济宁人,农艺师,硕士研究生,研究方向:果树育种与高效栽培研究工作。通信地址:255033 淄博市张店区和平街道商场西街197号,Tel:0533-2111645,E-mail:

  • 基金资助:
    中央引导地方科技发展资金项目“无花果良种高效栽培与精准采收及制干技术成果转化与应用示范”(YDZX2023098); 山东省重点研发计划农业良种工程“鲜食加工抗病无花果优新品种培育”(2023LZGC016)

Research Progress on Tissue Culture and Rapid Propagation of Ficus carica

FENG Shuaishuai1(), QIAO Feng1, JIA Ming2(), WEI Juan2, JIANG Tingting1, HUANG Ailing1, JIN Zhibo3, YANG Zong1, LI Aihua1, HAN Wei1, LI Quanxi1   

  1. 1 Zibo Academy of Agricultural Sciences, Zibo Shandong 255033
    2 Shandong Academy of Forestry, Jinan 250014
    3 Shandong New Coordinates Agricultural Development Co. Ltd., Zibo Shandong 256400
  • Received:2024-01-12 Revised:2024-06-26 Online:2025-02-20 Published:2025-02-18

摘要:

为提高无花果繁殖系数,满足推广无花果优良品种的需求,建立有效的无花果离体快繁体系,文章总结了部分无花果离体快繁体系的构建过程,包括外植体的建立、愈伤组织和不定芽的诱导分化、增殖培养和生根培养等阶段,分析了无花果在离体快繁过程中存在的组培苗污染、褐化、玻璃化等问题,并提出针对性的解决措施。同时展望了无花果快繁的应用前景,以期为无花果组培快繁体系的建立及进一步利用提供参考。

关键词: 无花果, 组织培养, 外植体, 愈伤组织

Abstract:

In order to increase the reproduction coefficient of fig (Ficus carica) plants, meet the demand for promoting excellent varieties of figs, and effectively establish an in vitro rapid propagation system for fig plants, this review focused on the progress in research on the fast breeding technology of fig plants. It summarized the construction process of in vitro rapid propagation system, including the establishment of explant cultures, the induction and differentiation of callus tissue and adventitious buds, proliferation culture, and rooting culture. The issues of contamination, browning, and vitrification encountered during the in vitro rapid propagation of fig plants were analyzed, along with targeted measures to address these problems. Additionally, prospects for its rapid propagation and application had been provided, to provide a reference for the establishment of a tissue culture system for fig plants and its further utilization.

Key words: Ficus carica, tissue culture, explant, callus