Abstract: In order to study SeFlammulina velutipes on human health issues. Using selenium-rich cultivation, automatic amino acid analyzer, phenol-sulfuric acid method Flammulina velutipes total sugar, amino acids the type were content measured study. The results showed that: Flammulina velutipes fruiting bodies contained total sugar, 17 kinds of amino acids and free amino acids, including 7 kinds of essential amino acids. Determining the optimum leaching selenium sugar temperature 80℃, extraction time 3 h, extracted 3 times the combined filtrate; total sugar pileus, stipe contents were 40.31%, 48.99%, selenium content of 34.2355 μg/g.