Abstract: The essential oil of fresh and dried banana bud was extracted by supercritical CO₂ fluid extraction technology, and their compositions and relative contents were quantitatively determined by GC-MS with peak areas normalization method. The result indicated that 13 and 14 compounds were identified from fresh and dried banana bud respectively, and 9 compounds were presented in both fresh and dried samples but the percentage contents were obvious difference. The major compositions of fresh banana bud were 9,19-Cycloergost-24(28)-en-3-ol,4,14 –dimethyl-,acetate,-(3á,4à,5à)- (49.04%), 1-Heneicosyl formate (10.84%), Tritetracontane (10.39%), 9-Octadecenamide,(Z)- (9.12%), Ethanol,2-(octadecyloxy)- (6.83%). The major compositions of dried banana bud were 9,19-Cycloergost-24(28) -en-3-ol,4,14 -dimethyl-,acetate,(3á,4à,5à)- (34.18%), 1-Heptacosanol (17.89%), 1-Heneicosyl formate (17.22%), Ethanol,2-(octadecyloxy)- (10.16%), 9-Octadecenamide,(Z)- (5.67%).