Abstract: In order to determine the disease resistance of different grape varieties to Colletotrichum gloeosporioide (Penz.) Sacc, the disease resistances were determined through the indoor inoculation. Then variance analysis and clustering analysis were done. The fruit hardness, soluble solid content and organic acid content were determined. The resistance of blade results by clustering analysis showed that‘Kyoho grape’and ‘Red Barbara’had the strongest resistance,‘Manicure finger’,‘Sun Rose Grape’,‘Wink’and‘Summer Black’showed resistance,‘Drunk the incense grape’,‘Zhaoping Red Grape’and‘Rosario Bianco’had little resistance to the disease,‘Red Milk’and‘Rosario Rosso’were poorly resistant to the disease. The resistance results of mature fruit by clustering analysis showed that‘Drunk the Incense Grape’,‘Manicure Finger’, ‘Eastern Star Grape’,‘Sun Rose Grape’showed resistance,‘Kyoho Grape’,‘Red Barbara’,‘Rosario Bianco’ had little resistance to the disease,‘Red milk’,‘Zhaoping Red Grape’,‘Rosario Rosso’and‘Summer Black’ were poorly resistant to the disease. The measure results of fruit hardness, soluble solid content and organic acid content showed that there were significant differences among different varieties, but these indexes had no significant correlation with the incidence of anthrax. Disease resistance of different grapes varieties to the anthracnose must be analyzed with consideration of grape varieties, physiological period, plant anagement mode, environment, climate and so on.