The Progress of Confocal Laser Scanning Microscope

doi:10.11923/j.issn.2095-4050.cjas16010025

Abstract

Abstract: Abstracts: At present, technique of the confocal laser scanning microscope is booming. This new technology aimed to improve the functions of the confocal laser scanning microscope from the following aspects: improve resolution, decrease the phototoxicity, and improve scanning speed, viviperception. Meanwhile, the potential of biology applications was great with the help of these new techniques, so it was necessary to sort out and analyze the principles of these new techniques. This essay mainly analyzed some techniques’ principles and potential in their applications, including Light gate, White laser, GaAsP, Ultrahigh-resolution, Live cell imaging system, Light-sheet ,Multi-photon microscopy, Coherent Anti-stokes Raman Scattering, Laser Capture Microdissection. Besides, the next research area and the shortcomings of the existing technique in scanning speed, compatibility, applicability of samples are also analyzed. This essay can assist researchers to learn more about some new techniques and make them know “what kind of technique they can use to achieve what kind of biology functions”in order that they can form more ideas of experiment research.

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