Welcome to Journal of Agriculture,

Journal of Agriculture ›› 2016, Vol. 6 ›› Issue (11): 64-66.doi: 10.11923/j.issn.2095-4050.cjas16050018

Special Issue: 生物技术 园艺

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桑树;DNA提取;PCR扩增

  

  • Received:2016-05-30 Revised:2016-08-11 Accepted:2016-08-25 Online:2016-11-25 Published:2016-11-25

Abstract: The aim is to explore an efficient, stable, low-cost DNA extraction method. The total genomic DNA was extracted from mulberry leaves with CTAB in Anhui. Ultraviolet spectrophotometer, agarose gel electrophoresis and PCR methods were used to test the DNA quality. The results showed that the value of A260/ A280 was 1.80-1.90, DNA yield was 0.187-0.275 μg/mg. The extracted DNA was used as templates, the clear target bands were obtained by PCR amplification. It indicated that CTAB method was suitable to extract the total genomic DNA from leaves of mulberry, which could meet the need of subsequent analysis of molecular biology.