Abstract: To detect pesticide residues in edible mushrooms, oyster mushroom samples were taken as the experiment material. The solid-phase extraction method was adopted to study the detection methods for 9 pesticide residues such as chlorothalonil and triadimefon in oyster mushrooms. The samples were extracted with acetonitrile, further cleaned-up by graphite carbon amino columns and detected by gas chromatography with electron capture detector (GC- ECD). The result showed that the relative standard deviations of nine pesticides content were 2.05%-6.35% under this method, the limits of detection were 0.0003-0.005 mg/kg and the limits of quantification were 0.0009-0.02 mg/kg. The nine pesticides had a good linearity from 0.05 to 2.0 mg/L and the r values were greater than 0.990. The method is feasible and accurate for determining nine residues in oyster mushroom.